Fig. 2

miR-214 attenuated NRK-52E cell apoptosis under hypoxia. a qRT-PCR analysis of miR-214 expression in kidney cortex of I/R-induced rat AKI models after 12, 48 and 72 h of reperfusion. n = 6 rats/group. b qRT-PCR analysis of miR-214 expression at 0, 3 h, 6 h, and 9 h during hypoxic incubation of cultured NRK-52E cells. c qRT-PCR analysis of miR-214 expression in NRK-52E cells after transfection with miR-214, anti-miR-214, or matched controls. d–e Flow cytometry was used to analyze the apoptotic rate of NRK-52E cells after transfection with miR-214, anti-miR-214, or respective controls during hypoxia incubation. f Western blot was performed to detect the protein levels of Bcl-2 and Bax in NRK-52E cells after introduction with miR-214, anti-miR-214, or corresponding controls under hypoxia. Each experiment was independently repeated 3 times. *P < 0.05