Fig. 5From: Knockdown of PAICS inhibits malignant proliferation of human breast cancer cell linesAssessment of apoptosis after PAICS knockdown in ZR-75-30 breast cancer cells. Apoptosis was investigated by flow cytometric analysis. a Representative diagrams showing the analysis of apoptosis. b The percentages of AnnexinV-APC/7-AAD-positive apoptotic cells are presented. c Cell cycle-related and apoptosis-related molecules were detected by western blotting using specific antibodies after PAICS knockdown in ZR-75-30 cells. GAPDH was used as the loading control. Data are shown as mean ± SD (n = 3; t test). ***p < 0.001Back to article page