Fig. 3
From: Melatonin ameliorates H2O2-induced oxidative stress through modulation of Erk/Akt/NFkB pathway
Expression level of Akt, Erk, NFkB (cytosolic), NFkB (nuclear) and melatonin receptor protein (MTR) in hepatocytes of Labeo rohita in control [Treatment group 1], H2O2 (12.5 µM) [Treatment group 2], H2O2 (25 µM) [Treatment group 3], H2O2 (50 µM) [Treatment group 4], H2O2 (50 µM) + melatonin (25 μg/ml) [Treatment group 5], H2O2 (50 µM) + melatonin (50 μg/ml) [Treatment group 6], H2O2 (50 µM) + melatonin (100 μg/ml) [Treatment group 7], melatonin (100 μg/ml) [Treatment group 8]. Panel on right side depicts representative bands for corresponding treatment groups. β-actin used as internal control. Notes: Akt protein kinase B, Erk extracellular signal regulated kinase, NFkB nuclear factor kappa B, MTR melatonin receptor. Akt, Erk and MTR measured only in cytosolic fraction. Data are means ± SE; (n = 6). Different alphabets indicate significant difference (P < 0.05) in mean values of corresponding parameter between different treatment groups. Treatment group with one particular letter (such as ‘d’) has significantly different mean value (P < 0.05) of the corresponding parameter than that in other treatment groups that show different letter (such as either ‘c’, ‘b’ or ‘a’)