Fig. 2

a Heat Shock Protein (HSP70 and HSP90) expression level in hepatocytes of Labeo rohita in control [Treatment group 1], H₂O₂ (12.5 µM) [Treatment group 2], H₂O₂ (25 µM) [Treatment group 3], H₂O₂ (50 µM) [Treatment group 4], H₂O₂ (50 µM) + melatonin (25 μg/ml) [Treatment group 5], H₂O₂ (50 µM) + melatonin (50 μg/ml) [Treatment group 6], H₂O₂ (50 µM) + melatonin (100 μg/ml) [Treatment group 7], melatonin (100 μg/ml) [Treatment group 8]. Panel on right side depicts representative bands for corresponding treatment groups. β-actin used as internal control. b Hydroxyl and Superoxide radical scavenging activity of melatonin at three different concentrations (25, 50 and 100 μg/ml) in hepatocyte of Labeo rohita. Notes: HSP heat shock protein. Data are means ± SE; (n = 6). Different alphabets indicate significant difference (P < 0.05) in mean values of corresponding parameter between different treatment groups. Treatment group with one particular letter (such as ‘d’) has significantly different mean value (P < 0.05) of the corresponding parameter than that in other treatment groups that show different letter (such as either ‘c’, ‘b’ or ‘a’)