Fig. 3

NKP608 causes inhibition of in HCT116 cells. a HCT116 cells apoptosis was tested by flow cytometric analysis after Annexin V-FITC/PI staining, and apoptotic cells (Annexin V+PI− and Annexin V+PI+) were shown. b Effect of NKP608 on the expression of Bax, Bcl-2 and Caspase-3 protein were detected by western blot assay. c Representative western blot images are shown. Values are expressed as the mean ± SD (n = 3). *p < 0.05 versus NC group