Fig. 6
From: MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma
Knockdown of ZNF346 suppresses cell proliferation and promoted apoptosis in NB cells. Cells were respectively transfected with the siZNF346 or si-NC, respectively for 48 h. a The expression of ZNF346 protein levels was determined by Western blot analysis in SH-SY5Y and SK-N-SH cells. b MTT assay was used to measure cell proliferation in SH-SY5Y and SK-N-SH cells. c, d Flow cytometry assay was performed to evaluate cell apoptosis in SK-N-SH andSH-SY5Y cells. e The protein expression of Caspase 3 and Bcl-2 were analyzed in SK-N-SH and SH-SY5Y cells infected with siZNF346 or si-NC by Western blot. GAPDH was used as the endogenous control. ***p < 0.001 versus si-NC