Fig. 7
From: Berberine ameliorates diabetic nephropathy by inhibiting TLR4/NF-κB pathway
Effects of TAK-242, or combined with BBR or/and PMA on inflammatory response and apoptosis in HG-induced podocytes. Podocytes were pre-treated with or without 1 μΜ TAK-242, or along with 100 ng/ml PMA for 2 h prior to stimulating with 30 mM high glucose. Then, cells were treated with 30 μM BBR for 24 h. The mRNA amounts of IL-1β (a), IL-6 (b), and MCP-1 (c) in treated podocytes were measured by western blot. d Flow cytometry analysis was performed to detect apoptotic rates of treated podocytes. e The protein levels of Cleaved Caspase-3 and Bcl-2 in treated podocytes were determined by western blot. f–i The protein levels of TLR4, p-IκBα, IκBα, p-p65, p65 in treated podocytes were determined by western blot. *P < 0.05, n = 3