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Fig. 4 | Biological Research

Fig. 4

From: HSP70 from the Antarctic sea urchin Sterechinus neumayeri: molecular characterization and expression in response to heat stress

Fig. 4

Protein detection of Sn-Hsp70 in S. neumayeri. a Validation of anti-Hsp70. Line 1: SDS-PAGE proteins profile of coelomocytes lysates stained with coomassie blue. Line 2: SDS-PAGE proteins profile stained with silver. Line 3: western blot for antibody validation show only a specific band (arrow) in the expected molecular weight (70 kDa), the antibody not present nonspecific recognition in other molecular weights. b Western blot to detect Sn-Hsp-70 in coelomocytes during the acute heat stress. c Indirect ELISA detection by Optical density to 450 nm of Sn-Hsp-70 protein in coelomocytes and digestive tract. Differences in Sn-Hsp70 detection are not significant by ELISA (P < 0.05)

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