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Fig. 3 | Biological Research

Fig. 3

From: HSP70 from the Antarctic sea urchin Sterechinus neumayeri: molecular characterization and expression in response to heat stress

Fig. 3

Expression analysis of Sn-Hsp70 mRNA. a Expression of Hsp70 in tissue by RT-PCR. b Sn-Hsp70 mRNA expression was measured by qPRC in coelomocytes following heat stress. Results are mean ± SE of three independent experiments carried out on a pool of four sea urchins from control group at 0.5 °C (black) and thermal stressed exposed to 5 °C (white) and 10 °C (grey) during 1, 24 and 48 h. The housekeeping gene used in the qPCR was the 28S rRNA. The primers efficiency was 112 and 109% for 28S rRNA and Sn-Hsp70, respectively

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