Fig. 6

Evaluation of hepatic NKT cells in wild type and PPARα^−/− mice. a Scatter plots of liver mononuclear cells stained for TCRβ (y axis) and NK cell markers (DX5 and NK1.1, x axis) from untreated wild type or PPARα^−/− mice. Representative scatter plots from 4 individual mice in each group are shown. Percentages represent those from total isolated liver mononuclear cells. b Serum alanine aminotransferase levels 0 or 12 h following administration of alpha-galactosylceramide (αGal) in wild type and PPARα^−/− mice. c Hepatic interferon gamma (IFNγ) gene expression 0 or 12 h following αGal administration in wild type and PPARα^−/− mice. *p < 0.05 vs. wild type at 0 h. ⁺p < 0.05 vs. wild type at 10 h post-injection. n = 3–4 animals per group