Fig. 4

G007-LK treatment reduced canonical WNT signalling. a, b Immunohistochemistry (IHC) of duodenal tissue sections stained with an antibody against β-catenin, from control (a) and G007-LK-treated, gavage 10 mg G007-LK/kg body mass (b) mice (black arrows indicate non-Paneth cells in the crypt base with positive nuclear β-catenin staining). c Quantification of the fraction of β-catenin-positive nuclei among the crypt base cells in control and G007-LK-treated mice (mean ± SEM, *p < 0,05, n = 3). d, e BrdU incorporation, after 1 h incubation, into crypt base and transit amplifying (TA) cells in control (d) and G007-LK-treated, enriched chow 100 mg G007-LK/kg chow (e) mice (positive cells indicated with black arrows). f Median ratios of BrdU positive cell in the crypt of control and G007-LK treated mice (Box = 25th and 75th percentile, bars = min and max values) All images were taken with a 40× objective (scale bar 20 μm)