Table 1 Primer sequences and associated PCR conditions
|  | Forward sequence | Reverse sequence | Product size (bps) | Annealing Tm (°C) |
|---|---|---|---|---|
HSP90-beta | TACTTGGTGGCAGAGAAAGT | CTCATCTGAACCCACATCTT | 441 | 60 |
RGS2 | CAAACAGCCGGGACTCCAGC | TGCTGGCATGCAGCTGGTCA | 303 | 63.5 |
RGS4 | TCCCTGGTCCCTCAGTGTGCC | AAGCATGCCCTGAGCACCCA | 958 | 64 |
PPARγ | AAGCCCTTCACTACTGTTGA | ACCTGATGGCATTATGAG AC | 444 | 56 |
CEBPα | CCTAAGGTTGTTCCCCTAGT | GAGAGTCTCATTTTGGCAAG | 547 | 58 |
LPL | GTCCGTGGCTACCTGTCATT | AGCCCTTTCTCAAAGGCTTC | 717 | 60 |
Runx2 | TCTTCACAAATCCTCCCC | TGGATTAAAAGGACTTGGTG | 230 | 55 |
OC | CTACCTGTATCAATGGCTG | CAGATTCCTCTTCTGGAGTTTA | 310 | 56 |
ALPL | TGGAGCTTCAGAAGCTCAACA | ATCTCGTTGTCTGAGTACCAG | 450 | 60 |
CDK1 | GGATCTACCATACCCATTGAC | CCATGTACTGACCAGGAGGG | 327 | 55 |
CDK2 | TGACTCGCCGGGCCCTATTCC | CCCAAGGCCAAGCCTGGTCA | 381 | 60 |
CDK4 | AGTTTCCGCGCGCCTCTTTG | GGCACAGACGTCCATCAGCCG | 455 | 63Â |
CCND1 | CTCCAGAGGGCTGTCGG | CTCGGCCGTCAGGGGGA | 528 | 60 |
Runx2/p57 | CGC CTC ACA AAC AAC CAC AG | TCA CTG TGC TGA AGA GGC TG | 225 | 60 |