Fig. 9

Effect of siRGS4 and siRGS2 on osteogenic differentiation of hMSCs. a Top row: Bright field images showing calcified deposits (yellowish color) in siCON and siRGS4 treatment groups. Middle row: Bright field images showing alizarin red stained cells with calcified deposits in red color in siCON and siRGS4 treatment groups. Bottom row: Bright field images showing alizarin red stained cells with calcified deposits in red color in siCON and siRGS2 treatment groups. b Alizarin red S stain quantification in siCON and siRGS4 treatment groups. c Alizarin red S stain quantification in siCON and siRGS2 treatment groups. Imges and graphs represent the mean quantification of siRGS treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS treatment groups. *p < 0.05