Fig. 8

Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI FBS CM based adipogenic media. Expression of PPARγ, C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p < 0.05