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Fig. 7 | Biological Research

Fig. 7

From: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

Fig. 7

Effect of siRGS4 and siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by Hyclone CM based adipogenic media. Expression of PPARγ, C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS4 (ac) or siRGS2 (df) transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS4 and siCON transfected cells. b Expression of C/EBPα in siRGS4 and siCON transfected cells. c Expression of LPL in siRGS4 and siCON transfected cells. d Expression of PPARγ in siRGS2 and siCON transfected cells. e Expression of C/EBPα in siRGS2 and siCON transfected cells. f Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p < 0.05, **p < 0.01

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