Fig. 7From: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cellsEffect of siRGS4 and siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by Hyclone CM based adipogenic media. Expression of PPARγ, C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS4 (a–c) or siRGS2 (d–f) transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS4 and siCON transfected cells. b Expression of C/EBPα in siRGS4 and siCON transfected cells. c Expression of LPL in siRGS4 and siCON transfected cells. d Expression of PPARγ in siRGS2 and siCON transfected cells. e Expression of C/EBPα in siRGS2 and siCON transfected cells. f Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p < 0.05, **p < 0.01Back to article page