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Fig. 6 | Biological Research

Fig. 6

From: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

Fig. 6

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p < 0.05

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