Fig. 4

Effect of siRGS4 on adipogenic differentiation of hMSCs induced by Hyclone CM based adipogenic media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Quantification of total nuclear count per treatment group. f Quantification of adipocyte count and percentage of adipocyte per treatment group. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON treatment wells from a representative experimental (n = 3). Comparison was made between siCON and siRGS4 treatment groups. *p < 0.05, **p < 0.01