Fig. 10

Effect of siRGS4 and siRGS2 on the expression of osteogenic markers. Expression of Runx2, OC and ALP were examined at day 1, 3, 7 and 14 post osteogenic treatment initiation in siCON and siRGS4 (a–c) or siRGS2 (d–f) transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. a Expression of Runx2 in siRGS4 and siCON transfected cells. b Expression of OC in siRGS4 and siCON transfected cells. c Expression of ALP in siRGS4 and siCON transfected cells. d Expression of Runx2 in siRGS2 and siCON transfected cells. e Expression of OC in siRGS2 and siCON transfected cells. f Expression of ALP in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS treatment groups at each given time point. *p < 0.05