Fig. 3

Cytokine levels in U937 cell cultures with 40% FFP. TNF, IL-1β, IL-10 and IL-6 were measured by ELISA in supernatants from cultured cells stimulated during 24 h solely with 40% FFP (panels a, c, e, g) or 40% FFP plus LPS (panels b, d, f, h). Assays were performed in triplicate. Bars represent means and whiskers denote standard error. Statistical analyses were performed using the Kruskal–Wallis test with multiple comparison post-test by Dunn. Analyses were 2-tailed, and a P <  0.05 value was used for significance