Fig. 3

Bcl-2 is the direct target of miR-181a. a Schematic representation of Bcl-2 mRNA with a putative 3ʹ-UTRmiR-181a-binding site and sequences of wildtype (Bcl-2 WT) and mutant (Bcl-2mut) miR-181a target sites. Seed region is framed; b Luciferase activity of 3ʹ-UTR-Bcl-2 (wt) was inhibited with Transfection Of miR-181a and such inhibition was not present in the miR-181a-binding site (mut) with mutations or with participation of miR-181a inhibitor. The influence of miR-181a on expression level of Bcl-2 was compared to the mimic negative control; c Western blotting analysis of Bcl-2 and β-actin protein levels after transfection of miR-181a or mimic NC, or miR-181a inhibitor or inhibitor NC. **P < 0.01