Table 1 Neurotransmitter tissue contents in EAAT3 HET and WT littermates determined by HPLC coupled to electrochemical (monoamines) and fluorometric (aminoacids) detection
Slc1a1 genotype | Glutamate (pg/mg tissue) | GABA (ng/mg tissue) | 5-HT (pg/mg tissue) | 5-HIAA (pg/mg tissue) | Dopamine (pg/mg tissue) | DOPAC (pg/mg tissue) |
|---|---|---|---|---|---|---|
Prefrontal cortex | ||||||
WT | 73.1 ± 9.0 n = 7 | 4605 ± 714.8 n = 6 | 525.4 ± 30.5 n = 7 | 581.3 ± 45.3 n = 7 | 35.2 ± 7.7 n = 6 | N.D |
HET | 67.7 ± 5.9 n = 7 | 4923 ± 338 n = 6 | 466.4 ± 37.2 n = 7 | 520.4 ± 33.0 n = 7 | 55 ± 12.9 n = 6 | N.D |
t-test | N.S. | N.S. | N.S. | N.S. | N.S. | |
Striatum | ||||||
WT | 100.9 ± 10.0 n = 7 | 6973 ± 463.3 n = 7 | 473.9 ± 51.5 n = 7 | 667.6 ± 76.8 n = 7 | 10,631 ± 1147 n = 6 | 2126 ± 370.6 n = 6 |
HET | 90.1 ± 11.4 n = 7 | 7658 ± 1372 n = 7 | 512 ± 51.8 n = 7 | 792.9 ± 72.3 n = 7 | 9593 ± 1296 n = 7 | 1818 ± 174.8 n = 7 |
t-test | N.S. | N.S. | N.S. | N.S. | N.S. | N.S. |
Thalamus | ||||||
WT | 67.4 ± 7.6 n = 7 | 13,449 ± 1714 n = 7 | 720.9 ± 64.5 n = 6 | 2078 ± 117.8 n = 5 | 227.9 ± 27.2 n = 7 | 55.3 ± 9.4 n = 6 |
HET | 49.6 ± 9.1 n = 7 | 11,785 ± 1965 n = 7 | 618.5 ± 113.4 n = 7 | 1821 ± 201.3 n = 7 | 183.1 ± 22.2 n = 7 | 72.2 ± 11.7 n = 6 |
t-test | N.S. | N.S. | N.S. | N.S. | N.S. | N.S. |