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Table 1 Neurotransmitter tissue contents in EAAT3 HET and WT littermates determined by HPLC coupled to electrochemical (monoamines) and fluorometric (aminoacids) detection

From: Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice

Slc1a1 genotype Glutamate (pg/mg tissue) GABA (ng/mg tissue) 5-HT (pg/mg tissue) 5-HIAA (pg/mg tissue) Dopamine (pg/mg tissue) DOPAC (pg/mg tissue)
Prefrontal cortex
 WT 73.1 ± 9.0
n = 7
4605 ± 714.8
n = 6
525.4 ± 30.5
n = 7
581.3 ± 45.3
n = 7
35.2 ± 7.7
n = 6
N.D
 HET 67.7 ± 5.9
n = 7
4923 ± 338
n = 6
466.4 ± 37.2
n = 7
520.4 ± 33.0
n = 7
55 ± 12.9
n = 6
N.D
 t-test N.S. N.S. N.S. N.S. N.S.  
Striatum
 WT 100.9 ± 10.0
n = 7
6973 ± 463.3
n = 7
473.9 ± 51.5
n = 7
667.6 ± 76.8
n = 7
10,631 ± 1147
n = 6
2126 ± 370.6
n = 6
 HET 90.1 ± 11.4
n = 7
7658 ± 1372
n = 7
512 ± 51.8
n = 7
792.9 ± 72.3
n = 7
9593 ± 1296
n = 7
1818 ± 174.8
n = 7
 t-test N.S. N.S. N.S. N.S. N.S. N.S.
Thalamus
 WT 67.4 ± 7.6
n = 7
13,449 ± 1714
n = 7
720.9 ± 64.5
n = 6
2078 ± 117.8
n = 5
227.9 ± 27.2
n = 7
55.3 ± 9.4
n = 6
 HET 49.6 ± 9.1
n = 7
11,785 ± 1965
n = 7
618.5 ± 113.4
n = 7
1821 ± 201.3
n = 7
183.1 ± 22.2
n = 7
72.2 ± 11.7
n = 6
 t-test N.S. N.S. N.S. N.S. N.S. N.S.
  1. Please see “Methods” section for further details. Data are expressed as mean ± SEM