Fig. 1

Reduced EAAT3 expression in EAAT3 heterozygous mice compared to wild-type littermates. a Representative Western Blot showing reduced EAAT3 protein expression in HET mice (H lanes) compared to WT mice (W lanes). b Graph bar of blot quantification; unpaired t-test P = 0.0076, n = 4 per group. c Real time qPCR detected significantly reduced EAAT3 and unaltered GLT-1 and GLAST mRNA levels in EAAT3 HET mice compared to WT littermates. Unpaired t-test with Welch´s correction (against WT mRNA levels): EAAT3 P = 0.0025; GLT-1 P = 0.9467, N.S.; GLAST P = 0.3980, N.S. n = 4 samples per group, each sample run in triplicate. ** indicates P<0.01