Fig. 2

MiR-320 regulates PBX3 expression in glioma cells. a miR-320 expression in U87 and U251 cells following transfection with miR-320 mimics or NC, and miR-320 expression was significantly increased in cells transfected with miR-320 mimics in comparison with that with NC. b PBX3 expression in U87 and U251 cells following transfection with miR-320 or NC, and PBX3 expression was significantly reduced by miR-320 compared with NC. c Western blot of PBX3 in U87 and U251 cells transfected with miR-320 mimics or NC, and protein expression of PBX3 was significantly reduced. d Computer prediction of miR-320 binding sites in the 3′UTR of PBX3 gene. Luciferase reporter assays that cells were transfected with 100 ng of wild-type 3′-UTR-reporter or mutant constructs with 100 nM of the miR-320 mimic or NC. ***P < 0.001, compared with negative control