Table 2 The primers for detection of PDCoV used in this study
Test | Primer ID | Type | Length (bp) | Sequence (5′–3′) |
|---|---|---|---|---|
RT-LAMP | PDCoV-F3 | Forward outer | 18 | CGGCTCTGCAGACACTGA |
PDCoV-B3 | Reverse outer | 19 | CCGTATTGAGCGCATCCTT | |
PDCoV-FIP | Forward inner | 42 | GCATTTCCTGAACACCAGGCACGAAGACGGGTATGGCTGATC | |
PDCoV-BIP | Reverse inner | 41 | CTGGCCACCTTGAGAGCAACTAGAACCCTCCTTGACTGTGA | |
RT-PCR | PDCoV-NF | Forward | 22 | CCAAACGCAACCCCAACAATCC |
PDCoV-NR | Reverse | 22 | CTTCTCAGTGTCTGCAGAGCCG | |
Nested RT-PCR | PDCoV-OF | Forward outer | 20 | TGCTACCTCTCCGATTCCCA |
PDCoV-OR | Reverse outer | 20 | ATCCTGTTTGTCTGCTGGCA | |
PDCoV-IF | Forward inner | 22 | GACACTGAGAAGACGGGTATGG | |
PDCoV-IR | Reverse inner | 22 | TAGTTGGTTTGGTAGGTGGCTC | |
Standard control | PDCoV-NWF | Forward | 21 | ATGGCCGCACCAGTAGTCCCT |
PDCoV-NWR | Reverse | 20 | CTACGCTGCTGATTCCTGCT |