Fig. 2From: A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplificationComparison of the sensitivity of the RT-LAMP, nested RT-PCR and conventional RT-PCR for PDCoV detection by agarose gel electrophoresis analysis (a RT-LAMP; b Nested RT-PCR; and c conventional RT-PCR) and by visual observation (d). M, DL500 DNA marker; lane 1–10: a serial of diluents of the recombinant plasmid containing partial PDCoV N gene insert, i.e., 1.0 × 108, 1.0 × 107, 1.0 × 106, 1.0 × 105, 1.0 × 104, 1.0 × 103, 1.0 × 102, 1.0 × 101, 1.0 × 100 copies, and negative controlBack to article page