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Fig. 4 | Biological Research

Fig. 4

From: Neuron-derived CCL2 contributes to microglia activation and neurological decline in hepatic encephalopathy

Fig. 4

Neuron-derived CCL2 induced the proliferation and activation of microglia. After stimulation with 10 ng/ml TNF-α for 10 min and washed with PBS, neurons were cultured in fresh medium for 3 h. Then the medium and lysates (CM) were collected and added into microglia cultures. To block CCL2, the microglia was pretreated with 5 μM CCL2 receptor inhibitor (INCB or C021) for 1 h before the addition of CM. After incubation for 24 h, the expression of Iba1 was determined using qRT-PCR (a) and western blotting (b, c). The proliferation of microglia was measured using CCK8 assay (d). **P < 0.01, *P < 0.05, vs Ctrl; ## P < 0.01, # P < 0.05, vs CM

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