Fig. 4

The p38 MAPK signaling pathway was involved in TMPyP4-inhibited cell proliferation in human cervical cancer cells. Cells were seeded in 6-well plates for 48 h and then treated with SB203580 in human cervical cancer cells for 24 h, Western blot was performed to determine protein expression of p-p38 MAPK and MK-2 for evaluating the blocking efficiency of SB203580 (a). Cells were treated with SB203580 for 24 h, and 20 μM TMPyP4 was added for 24 h, then cell viability was determined by MTT method (b). Data are expressed as mean ± SD of three independent experiments in six replicates. *P < 0.05 or **P < 0.01 indicates significance, P > 0.05 means no difference