Table 1 Primer sequences used in this study
From: Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary
Primer name | Sequence (5′ to 3′) | Tm (°C) | Size (bp) |
|---|---|---|---|
RT-PCR | |||
CART-F | AGCGCGGCTCGGCGGGATTCGGCAGC | 58.7 | 396 |
CART-R | GGGCGGACGTGCACCGCGCCGGTGCC | 58.7 | |
qRT-PCR | |||
CART-R-F | GAGAAGGAGCTGATCGAGGC | 60.0 | 88 |
CART-R-R | CCTGCCCGAACTTCTTTTCG | 59.5 | |
β-actin-F | AATGGCTCCGGTATGTGCAA | 60.0 | 112 |
β-actin-R | GGCCCATACCAACCATCACA | 60.0 | |