Fig. 4
Depletion of USP39 induces G2/M phase cell cycle arrest in U2OS cells. U2OS cells were inoculated into 6-cm dishes at a density of 2 × 105 cells per dish for cell cycle test and 80,000 cells per dish for apoptosis after 40 h lentivirus infection. a Cell cycle distribution of U2OS cells was analyzed by flow cytometry. b The population of cells in the G2/M phase was remarkably increased, accompanied by a noticeable reduction in the S phase (Student’s t test, n = 3). c USP39 silencing resulted in a cell cycle arrest via a cyclin-dependent way. d Statistic analysis of results in western blot (Student’s t test, n = 3). ***p < 0.001