Fig. 2

Embryoid bodies (EBs) at different stages of differentiation. a Day 5 of differentiation, mouse pluripotent stem cells (mPSCs) on differentiation began to form cell aggregates, called EBs with a diameter of 52.493 ± 0.849 µm. b Day 10 of differentiation, cell density of EBs growth, as well as EBs diameters, presenting a diameter of 156.28 ± 11.410 µm. c, d Day 15 of differentiation, dense EBs were shown with a diameter of 256.934 ± 15.852 µm. d The three-dimensionality of the EBs is evidenced. e EB on day 20 of differentiation. The EBs on this stage significantly increased in size, presenting a diameter of 1651.39 ± 32.96 µm. A reconstruction by tilling fields was made. Results were expressed as mean ± SD (×100, p < 0.05)