From: Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D cultures
S. no | Cell line/culture type | 1H NMR markers (ppm) | Assignment | Reference | |
---|---|---|---|---|---|
1 | 2D | Primary melanoma | 5.86 (d) | Guanosine | [21] |
2 | Metastatic melanoma | – | – | – | |
3 | 3D | Primary melanoma | 3.58 (d) | Threonine | |
3.60 (d) | Threonine | [24] | |||
3.67 (s) | Glucose | [23] | |||
3.75 | Glutamine | ||||
4.12 (t) | Glycerophospoethanolamine | ||||
4.39 (s) | N-Acetylgalactosaminitol | [27] | |||
6.16 (d) | Glucose | [28] | |||
8.07 (s) | NH2 | [29] | |||
4 | Metastatic melanoma | 8.25 (s) | ATP | [30] | |
5 | Primary melanoma | 2D | 1.16 (s) | Lipids | [31] |
2.71 (s) | Dimethylamine | [32] | |||
2.74 (s) | Dimethylamine | [33] | |||
3.04 | Creatine/phosphocreatine | [25] | |||
3.25 (s) | Choline head groups of phospholipids | [36] | |||
6 | 3D | 2.19 (s) | Adipate | [37] | |
2.72 (t) | Unassigned | ||||
2.93 (s) | Dimethyl glycine | [26] | |||
3.58 (d) | Threonine | [23] | |||
3.61 (d) | Glycerol | [38] | |||
4.02 (s) | 2 Hydroxyglutarate | ||||
4.12 (t) | Glycerophosphoethanolamine | [38] | |||
4.39 (s) | N-Acetylgalactosaminitol | [27] | |||
6.07 (d) | Unassigned | ||||
6.17 (s) | Unassigned | ||||
7 | Metastatic melanoma | 2D | 3.08 | Methyl lysine | [40] [41] |
3.72–3.75 (q) | Unassigned | ||||
3.90 (s) | Glucose | [42] | |||
8 | 3D | 3.19 (s) | O-Acetylcarnitine | [37] | |
3.48 (s) | Unassigned | ||||
9 | 2D | 2.85 (s) | Asparagine | [43] | |
3.22 | Choline | [25] | |||
3.96 (t) | β-Cyclodextrin | [44] | |||
5.29 (s) | Hc–ch (l, ch) | [31] | |||
10 | 3D | 2.72 (t) | Unassigned | ||
3.63 (s) | Glycerol | [38] | |||
3.66 | Glycerol/inositol | [38] [45] | |||
3.78 | Phosphoinositol | [45] | |||
4.05 (t) | Ethanolamine | [45] | |||
4.21 | Phosphocholine | [38] | |||
4.32 | Glycerophosphocholine | [31] | |||
7.12 (s) | Ar–H | [31] | |||
7.44 (d) | Unassigned | ||||
7.83 (s) | Amide (–NH) | [37] |