Fig. 3
Immunohistochemistry results in testis. a Immunolocalization of StAR in the testis in the breeding seasons. b Immunolocalization of P450scc in the testis in the breeding seasons. c Immunolocalization of 3β-HSD in the testis in the breeding season. d Immunolocalization of StAR in the testis in the non-breeding seasons. e Immunolocalization of P450scc in the testis in the non-breeding seasons. f Immunolocalization of 3β-HSD in the testis in the non-breeding season. LC Leydig cells, Spg spermatogonium, pSpc primary spermatocyte, rSpd round spermatid, eSpd elongated spermatid. g The immunodetection rate of StAR, P450scc and 3β-HSD in the breeding season were all significantly higher than in the non-breeding season (0.01 < p < 0.05; **p < 0.01). h Testosterone in the serum samples was assayed by ELISA three times. The left bar represents the average testosterone concentration in the breeding season (1.874 ± 0.167 ng/ml). The right bar represents the average testosterone concentration in the non-breeding season (0.198 ± 0.067 ng/ml). The seasonal difference between the average serum testosterone concentrations was significant (p < 0.01). (**p < 0.01)