Fig. 5
BJ-1108 ameliorates the onset and development of experimental autoimmune encephalomyelitis (EAE) by attenuating the generation of Th1 and Th17 cells. Acute EAE was induced in 8- to 12-week-old C57BL/6 mice by subcutaneous immunization with MOG35–55 in complete Freund’s adjuvant and pertussis toxin. Mice were administered 1 mg/kg BJ-1108 or vehicle intraperitoneally each day. a Clinical scores were assigned daily. b Total cell count in spleen and CNS of drug treated and untreated EAE mice. c Twenty-four days later, total mononuclear cells were isolated from brains and spinal cords of mice and analyzed by flow cytometry. Total percent of infiltrated CD4+ T cells, CD8+ T cells CD11+ cells and B220+ cells in CNS. d 24 days later, lymphocytes from spleen, LNs, and spinal cords were re-stimulated with phorbol 12-myristate 13-acetate and ionomycin for 4 h, followed by measurement of IFN-γ- and IL-17A-producing CD4+ T cells using flow cytometry. Numbers in the dot plots represent percentages of Th1 and Th17 cells. The mean ± SEM of five independent experiments is shown. *p < 0.01 and **p < 0.001 versus vehicle