Fig. 4

Suppression of inflammation in vivo by BJ-1080 in complete Freund’s adjuvant/ovalbumin (CFA/OVA)-immunized mice. Acute inflammation was induced in 8- to 12-week-old C57BL/6 mice by intraperitoneal immunization with OVA in CFA, and then 1× PBS or 1 mg/kg BJ-1108 was administered intraperitoneally each day. a Images of spleens, lymph nodes, and draining lymph nodes (dLNs) from CFA/OVA-immunized mice treated or untreated with BJ-1108 after 4 days. CD4⁺ T cells from b dLNs and c spleens were re-stimulated with phorbol 12-myristate 13-acetate and ionomycin for 4 h, followed by measurement of IFN-γ- and IL-17A-producing CD4⁺ T cells by flow cytometry. Numbers in the dot plots represent percentages of Th1 and Th17 cells. The mean ± SEM of five independent experiments is shown. ^# p < 0.01 versus vehicle. *p < 0.01 and **p < 0.001 versus CFA/OVA-treated group