Fig. 1
From: Metabotropic glutamate receptor 5 may be involved in macrophage plasticity
Expression of mGluR5, EAAT-2, PPAR-γ and HMGB1 proteins in control and mGluR5-transfected macrophages. RAW 264.7 cells (RAW-NT) and mGluR5-transfected macrophages (RAW-mGluR5) (5–105 cells per well) were incubated with LPS (100 ng ⁄ ml) or IL-10 (20 nM) for 24 h, followed by the determination of EAAT-2 (b), PPAR-γ (d) and HMGB1 (f) expression by western blot analysis, as described in the “Methods” section. (h) β-Actin was also visualized by Western blotting to confirm equal loading of the fractions. Data shown are representative of three independent experiments. Quantification of EAAT2 blots shown in c, *P < 0.05, vs corresponding RAW-NT cells. Quantification of PPAR-γ shown in e, *P < 0.05, vs corresponding RAW-NT cells, **P < 0.05, vs mGluR5 control. Quantification of HMGB1 blots shown in g, *P < 0.05, vs corresponding RAW-NT cells