Fig. 2

Uptake of ISONA by Huh7 and Hep3B cells with or without FuGENE The radioactivity was visualized by either dot blot (a) or quantified by scintillation counter (b). ISONA150 was added to Hep3B (l, 2, 3) and Huh7 [4–6]. Lines 1 and 4: 6 µl of 2 µg ISONA150 without FuGENE; lines 2 and 5: 6 µl of 2 µg ISONA150 + 3 µl FuGENE; lines 3 and 6: 6 µl of 1 µg ISONA150 + 4 µl FuGENE. In the third well of line 5: 1 µl of 0.33 µg ISONA + 4 µl FuGENE. Line 4 of Huh 7 cells had strong uptake even without FuGene added. The well of line 5 with less DNA probe showed much lower uptake compared with others of 4, 5 and 6