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Fig. 1 | Biological Research

Fig. 1

From: Uptake of DNA by cancer cells without a transfection reagent

Fig. 1

Uptake of DNA fragments with different size by HCC cells versus normal liver cells. a Different size of AFP cDNA fragments were prepared with PCR (57, 150, 310, 570 and 1620 bp). b After incubation with the cells overnight, radioactivity was visualized by dot blot (the top picture); triplicate wells were used to test each DNA fragment in the two cell lines. The radioactivity was also quantified by scintillation counter (the lower picture). The uptake is very strong in HCC cells but is minimal in normal liver cells. There is at least a tenfold difference

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