Fig. 4

BAY61 attenuates VSMC outgrowth from endothelium denuded aortic ring. To examine the effect of BAY61 on the outgrowth of VSMCs from blood vessel, segments of endothelium-denuded aortic rings embedded in Matrigel were treated with either vehicle (DMSO 2%, v/v) or BAY61 (10 μM) for 7 days. On day 7, the pictures of VSMC sprouting from the aortic ring were taken. White arrows indicate the sprouting of VSMCs from the aortic rings. *p < 0.05. The quantitative data were expressed as the mean ± SEM of at least three independent experiments