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Fig. 3 | Biological Research

Fig. 3

From: Klotho sensitive regulation of dendritic cell functions by vitamin E

Fig. 3

Effect of VitE on ROS formation. a Representative FACS histograms depicting ROS-dependent DCFDA fluorescence in control-(1st panel) and klotho-silenced (2nd panel) DCs are untreated (gray filled) or treated with LPS in the absence (solid line) or presence (dotted line) of VitE. b Arithmetic mean ± SEM (n = 5) of ROS production in DCs is shown prior to control (white bar) and 24 h following treatment with LPS (100 ng/ml) either in the absence (2nd bar) or presence of VitE (3rd bar) or presence of VitE and Bay 11-7082 (10 µM) (4th bar). c Arithmetic mean ± SEM (n = 5) of ROS production in DCs transfected with klotho siRNA is shown prior to control (1st panel) and 24 h following treatment with LPS (100 ng/ml) either in the absence (2nd bar) or presence (3rd bar) of VitE. *(p < 0.05) represents significant difference from LPS-stimulated DCs (ANOVA)

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