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Fig. 1 | Biological Research

Fig. 1

From: Klotho sensitive regulation of dendritic cell functions by vitamin E

Fig. 1

Effect of VitE on klotho expression. a Original Western blot of DCs were either treated with LPS (100 ng/ml) in the presence or absence of VitE (500 µM, 2 h) or left untreated (control). Protein extracts were analyzed by direct Western blotting using antibodies directed against p-IκBα and GAPDH. b Arithmetic mean ± SEM (n = 4) of the abundance of p-IκBα protein as the ratio of p-IκBα/GAPDH. c Arithmetic mean ± SEM (n = 5) of klotho transcript level is shown prior to control (white bar) and 5 h following incubation with VitE (500 µM) either in the absence (black bar) or presence of NF-κB inhibitor Bay 11-7082 (10 µM, grey bar). d Immunoprecipitation of klotho in DC supernatants is shown prior to control (1st panel) and 5 h following treatment with VitE (500 µM) either in the absence (2nd panel) or presence (3rd panel) of Bay 11-7082 (10 µM). Immunoprecipitates were stained for klotho and GAPDH antibodies. e Arithmetic mean ± SEM (n = 5) of the abundance of klotho protein as the ratio of klotho/GAPDH. *(p < 0.05) represent significant difference from control DCs, and #(p < 0.05) indicates significant difference from VitE-treated DCs (ANOVA)

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