Fig. 6

Effects of SE on mucus production in lung tissue. a Histological examination of mucus secretion in lung tissue was performed 24 h after the last OVA challenge. Lung tissues were fixed, sectioned at 4-µm thickness, and stained with periodic acid Schiff (PAS) reagent to assess mucus production (magnification ×100 and 200). b Scoring of mucus was quantified according to the percentage of PAS-positivity among all epithelial cells. Values are expressed as mean ± SE (n = 8). Means with the same letters are not significantly different at P < 0.05 using Duncan’s multiple-range test