Fig. 3
From: Upregulation of MiR-196a promotes cell proliferation by downregulating p27kip1 in laryngeal cancer
miR-196a directly targets the p27kip1 gene. a The luciferase reporter plasmid containing wild-type or mutant p27kip1 3′-UTR was cotransfected into HEK-293T cells with miR-196a mimic or mimic NC. Luciferase activity was determined by the dual luciferase assay and shown as the relative firefly activity normalized to Renilla activity. b The level of p27kip1 mRNA was determined by qPCR. c the expression of p27kip1 protein was analyzed by Western blotting. β-actin was used as control. *p < 0.05; **p < 0.01