Improved assembly procedure of viral RNA genomes amplified with Phi29 polymerase from new generation sequencing data

Table 3 Percentage of reads which unmapped on contigs generated with different assemblers

		Middelburg ArTB 5290	Mengovirus AnrB 3741	Mengovirus ArB 19017	p value^a
Abyss	Targeted sequences	6.99 %	2.64 %	2.25 %	0.04
Abyss	Untargeted sequences	40.59 %	36.93 %	69.78 %	0.04
Ray	Targeted sequences	6.76 %	0.92 %	2.26 %	0.14
Ray	Untargeted sequences	53.84 %	51.5 %	71.41 %	0.14
SPAdes 3.0	Targeted sequences	0.57 %	0.82 %	0.65 %	0.001
SPAdes 3.0	Untargeted sequences	47.39 %	55.87 %	60.02 %	0.001
SPAdes 3.5/3.6	Targeted sequences	3.05 %	0.6 %	0.48 %	0.02
	Untargeted sequences	34.12 %	55.54 %	60.02 %	0.02
p value^a		0.13	0.003	0.01	5.10e−6

ISSN: 0717-6287