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Table 2 Assembly of Mengovirus and MIDV genomes with different assembler software with targeted and untargeted sequences obtained after selection using similarity-based approach

From: Improved assembly procedure of viral RNA genomes amplified with Phi29 polymerase from new generation sequencing data

 

ArTB 5290

ArB 19017

AnrB 3741

Targeted

Untargeted

Targeted

Untargeted

Targeted

Untargeted

ABYSS

Contigs (≥50 bp)

2463

11,822

24

20,618

15

5905

Contigs (≥1000 bp)

3

0

2

0

1

0

Largest contig

1807

372

3219

195

7025

266

Mean length

88

84

576

82

392

82

N50

1185

124

2574

124

7025

124

L50

3

1501

1

2218

1

599

Ray

Contigs (≥50 bp)

2

35

3

12

4

28

Contigs (≥1000 bp)

2

0

2

2

2

0

Largest contig

6906

837

4088

1873

4360

552

Mean length

4844

284

2013

210

2571

523

N50

6906

575

4088

1102

3745

552

L50

1

2

1

2

1

1

SPAdes v3.0

Contigs (≥50 bp)

2

371

1

16

1

322

Contigs (≥1000 bp)

1

2

1

2

1

0

Largest contig

11,468

1065

7548

3492

7562

951

Mean length

5789

145

7562

127

7548

110

N50

11,468

892

7548

3492

7562

951

L50

1

3

1

1

1

1

SPAdes v3.5/SPAdes v3.6

Contigs (≥50 bp)

5

10,435

7

569

2

322

Contigs (≥1000 bp)

1

0

1

0

1

0

Largest contig

11,314

331

7548

396

7548

951

Mean length

2359

100

3896

127

1174

110

N50

11,314

129

7548

141

7548

951

L50

1

2492

1

113

1

1

  1. The reads whose regions of the viral sequences were selected within the reads were named «Targeted Sequences» or TS, whereas the untreated sequences were named «Untargeted Sequences» or US