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Fig. 3 | Biological Research

Fig. 3

From: Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro

Fig. 3

Laminin production by TEC after GH-treatment. TECs were plated in labtek chamber slides, treated with GH (100 ng/mL) for 24 h and then analyzed by fluorescence microscopy. a Photomicrographs show the production of laminin ascertained by immunofluorescence and fluorescence microscopy analysis. b Bars correspond to the quantitative analysis of laminin production in TEC in selected microscopic fields. Results are expressed as pixels/μm2. GH-treated cells increase laminin deposition. c Cytofluorometric profiles of TEC immunolabeled with anti-CD49f mAb, which defines the alpha chain of integrin VLA-6, the main receptor for laminin. Filled curves represent positive cells for VLA and white curve represents the Ig isotype control. Values correspond to mean ± SEM of three independent experiments, **p ≤ 0.01

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