TY - JOUR AU - Traipe-Salas, Felipe AU - Traipe-Castro, Leonidas AU - Salinas-Toro, Daniela AU - López, Daniela AU - Valenzuela, Felipe AU - Cartes, Christian AU - Toledo-Araya, Héctor AU - Pérez, Claudio AU - López Solís, Remigio PY - 2016 DA - 2016/06/03 TI - Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques JO - Biological Research SP - 28 VL - 49 IS - 1 AB - Tear desiccation on a glass surface followed by transmitted-light microscopy has served as diagnostic test for dry eye. Four distinctive morphological domains (zones I, II, III and transition band) have been recently recognized in tear microdesiccates. Physicochemical dissimilarities among those domains hamper comprehensive microscopic examination of tear microdesiccates. Optimal observation conditions of entire tear microdesiccates are now investigated. One-μl aliquots of tear collected from individual healthy eyes were dried at ambient conditions on microscope slides. Tear microdesiccates were examined by combining low-magnification objective lenses with transmitted-light microscopy (brightfield, phase contrasts Ph1,2,3 and darkfield). SN - 0717-6287 UR - https://doi.org/10.1186/s40659-016-0089-0 DO - 10.1186/s40659-016-0089-0 ID - Traipe-Salas2016 ER -