TY - JOUR AU - Nguyen, T. AU - Staines, D. AU - Nilius, B. AU - Smith, P. AU - Marshall-Gradisnik, S. PY - 2016 DA - 2016/05/31 TI - Novel identification and characterisation of Transient receptor potential melastatin 3 ion channels on Natural Killer cells and B lymphocytes: effects on cell signalling in Chronic fatigue syndrome/Myalgic encephalomyelitis patients JO - Biological Research SP - 27 VL - 49 IS - 1 AB - Transient receptor potential melastatin 3 (TRPM3) cation channels are ubiquitously expressed by multiple cells and have an important regulatory role in calcium-dependent cell signalling to help maintain cellular homeostasis. TRPM3 protein expression has yet to be determined on Natural Killer (NK) cells and B lymphocytes. Multiple single nucleotide polymorphisms have been reported in TRPM3 genes from isolated peripheral blood mononuclear cells, NK and B cells in Chronic fatigue syndrome/Myalgic encephalomyelitis (CFS/ME) patients and have been proposed to correlate with illness presentation. The object of the study was to assess TRPM3 surface expression on NK and B lymphocytes from healthy controls, followed by a comparative investigation examining TRPM3 surface expression, and cytoplasmic and mitochondrial calcium influx in CD19+ B cells, CD56bright and CD56dim cell populations from CFS/ME patients. SN - 0717-6287 UR - https://doi.org/10.1186/s40659-016-0087-2 DO - 10.1186/s40659-016-0087-2 ID - Nguyen2016 ER -