The expression and purification of rTCS. a PCR amplification of CDS for rTCS. Lane 1 stands for the CDS coding rTCS. b Expression of rTCS in BL21 E. coli.
Lane 1 to lane 3 stands for the control, clone 1, clone 2 respectively. c Purified rTCS by affinity adsorption. Lane 1 stands for purified rTCS. D. Western blot analysis of rTCS with anti-TCS antibody. Lane 1 and lane 2 stand for the same sample