|
Samples
|
Conc. 31. 25 µg/ml
|
Conc. 62.5 µg/ml
|
Conc. 125 µg/ml
|
Conc. 250 µg/ml
|
Conc. 500 µg/ml
|
Conc. 1000 µg/ml
|
IC50 µg/ml
|
|---|
|
Rh.Cr
|
39. 3 ± 0.7***
|
43.5 ± 0.6***
|
48.7 ± 0.8***
|
54.1 ± 0.2***
|
62.4 ± 0.3***
|
71.3 ± 1.3***
|
164.3
|
|
Rh.Hex
|
27.3 ± 1.3***
|
32.9 ± 0.5***
|
36.7 ± 0.8***
|
41.9 ± 0.3***
|
43.0 ± 0.2***
|
47.2 ± 1.1***
|
1011.5
|
|
Rh.Chf
|
56.7 ± 1.6*
|
59.3 ± 0.6**
|
64.7 ± 1.6
|
68.3 ± 0.5*
|
74.3 ± 1.2*
|
81.6 ± 1.7*
|
17.9
|
|
Rh.EtAc
|
43.3 ± 0.6***
|
49.6 ± 0.8**
|
55.3 ± 0.3**
|
61.0 ± 1.2***
|
67.3 ± 0.7***
|
72.3 ± 1.2***
|
65.5
|
|
Rh.Aq
|
11.6 ± 1.7
|
16.0 ± 1.1
|
23.3 ± 1.2
|
33.3 ± 0.3
|
37.0 ± 1.6
|
46.3 ± 0.4
|
1034.2
|
|
Rh.Sp
|
43.4 ± 0.9***
|
49.1 ± 1.4**
|
54.0 ± 0.6**
|
67.9 ± 0.5**
|
71.4 ± 0.3***
|
78.9 ± 1.0**
|
64.9
|
- Dexamethasone was used as positive control with IC50 value of 11.68 µg/ml; data is represented as mean ± SEM (Standard Error Mean); * <0.05, ** <0.01, *** <0.001
-
Rh.Cr crude methanolic extract, Rh.Hex
n-hexane fraction, Rh.Chf chloroform fraction, Rh.EtAc ethyl acetate fraction, Rh.Aq aqueous fraction, Rh.Sp saponins
