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Fig. 3 | Biological Research

Fig. 3

From: Wnt-5a-regulated miR-101b controls COX2 expression in hippocampal neurons

Fig. 3

Wnt-5a treatment increases the expression of COX2 in a time-dependent manner. a WB analysis of COX2 levels in hippocampal neurons treated with recombinant Wnt-5a (300 ng/mL) at different time points. b Densitometric analysis of the WB experiments shown in a. The results are presented as the mean of n = 5 experiments, and the statistical analysis was performed using one-way ANOVA, followed by post hoc Bonferroni’s analysis *p < 0.05 c Immunofluorescence analysis of COX2 expression in hippocampal neurons. Upper panel Representative images of control hippocampal neurons at 14 DIV. Lower panel Representative images of hippocampal neurons treated with rWnt-5a (300 ng/mL) for 1 h at 14 DIV (COX2 Blue, β-III-Tubulin green. White bar corresponds to 20 μm). d Quantification of somatic mean fluorescence intensity. n = 3, and the statistical analysis was performed using the Mann-Whitney U test * p < 0.05. e Analysis of COX2 mRNA expression using quantitative real-time PCR in hippocampal neurons treated for 1 h with rWnt-5a (300 ng/mL) at 14 DIV. The results are normalized to GAPDH expression and represented as the mean of n = 3 experiments. The statistical analysis was performed using the Mann–Whitney U test (NS, no significant changes)

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